In this lesson on “Signal Integrity Challenges in Multi-Die Systems”, we will explore the critical role of maintaining clean, accurate, and timely signal transmission in electronic systems, using relatable examples like conversations disrupted by noise or distractions. The lesson introduces the concept of Signal Integrity, highlighting challenges such as noise, signal reflections, timing issues, and crosstalk, which can lead to data corruption and degraded system performance. It also examines tools like eye diagrams to visualize signal quality and emphasizes the importance of electromagnetic simulation for accurate analysis, particularly in high-speed and multi-die systems. The lesson concludes by addressing the unique challenges of multi-die assemblies, including electromagnetic coupling, impedance mismatches, power-induced noise, and thermal effects, and emphasizes the need for co-optimization of signal and power integrity to ensure reliable operation in advanced 2.5D/3D-IC designs.